human brain tumor tissue array slide  (Pantomics Inc)

Journal: Oncotarget

Article Title: Downregulation of BRCA1-BRCA2-containing complex subunit 3 sensitizes glioma cells to temozolomide

doi:

Figure Lengend Snippet: Human brain tissue slide used for this study contained 24 cases of patients with different grades of gliomas in duplicates. The tissue slide was subjected to immunohistochemistry staining using anti-BRCC3 antibody (Abcam). The representative images show BRCC3 immunoreactivity in normal human cortical tissue (A) grade I astrocytoma (B) grade II astrocytoma (C) grade III anaplastic astrocytoma (D) grade IV glioblastoma multiforme (E) . Experiments were repeated using anti-BRCC3 antibody from ProSci with similar observations. The staining was photographed under microscope with four images taken from each case. BRCC3 immunoreactivity of normal brain tissue and different grades of glioma were evaluated using ImageJ software (F) . Cells with BRCC3 immunostaining were selected through threshold setting of ImageJ software. The data are referred as immunoreactivity score (IRS) representing the average intensity of BRCC3-positive cells normalized over the intensity of background. ** p < 0.01, versus normal tissue. Scale bar in A-E, 100 μm.

Article Snippet: Human brain tumor tissue slide (Pantomics Inc., Richmond, CA, USA) was first heated at 60 ° C for 30 min before deparaffinization using xylene.

Techniques: Immunohistochemistry, Staining, Microscopy, Software, Immunostaining